Effects of Orange Leaves Extraction Conditions on Antioxidant and Phenolic Content: Optimization Using Response Surface Methodology
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Uysal, SengulCvetanović, Aleksandra
Zengin, Gokhan
Đurović, Saša
Zeković, Zoran
Aktumsek, Abdurrahman
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In the last few years, bioactive components or their extraction techniques are gaining special interest in scientific areas. In this framework, orange leaves were used for preparation of extracts with high content of biologically active compounds. To optimize the extraction process, three levels and three variables of Box-Behnken design with response surface methodology were applied. Investigated responses were the total phenolic content (TPC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, cupric ion reducing antioxidant capacity (CUPRAC), and ferric reducing antioxidant power (FRAP). Independent variables were methanol concentration (10-90%), temperature (20-60 degrees C), and extraction time (60-180min). Experimentally obtained results were fit into a second-order polynomial model with multiple regression. Analysis of variance was used to estimate model fitness and determine optimal conditions for processing. Estimated optimal conditions were 90% methanolic solution, 60 degrees C and... 180min using these parameters; the predicted values of investigated responses were 43.19mg GAE/g (GAE: gallic acid equivalents), 43.04mg TE/g (TE: trolox equivalents), 139.34 and 93.76mg TE/g for TPC, DPPH, CUPRAC, and FRAP, respectively. The obtained optimal conditions could be considered as an alternative strategy for developing novel functional products.
Keywords:
response surface methodology / orange leaves / optimization / extraction process / Box-Behnken designSource:
Analytical Letters, 2018, 51, 10, 1505-1519
DOI: 10.1080/00032719.2017.1382501
ISSN: 0003-2719
WoS: 000429348300005
Scopus: 2-s2.0-85044028269
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Institut za opštu i fizičku hemijuTY - JOUR AU - Uysal, Sengul AU - Cvetanović, Aleksandra AU - Zengin, Gokhan AU - Đurović, Saša AU - Zeković, Zoran AU - Aktumsek, Abdurrahman PY - 2018 UR - https://riofh.iofh.bg.ac.rs/handle/123456789/597 AB - In the last few years, bioactive components or their extraction techniques are gaining special interest in scientific areas. In this framework, orange leaves were used for preparation of extracts with high content of biologically active compounds. To optimize the extraction process, three levels and three variables of Box-Behnken design with response surface methodology were applied. Investigated responses were the total phenolic content (TPC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, cupric ion reducing antioxidant capacity (CUPRAC), and ferric reducing antioxidant power (FRAP). Independent variables were methanol concentration (10-90%), temperature (20-60 degrees C), and extraction time (60-180min). Experimentally obtained results were fit into a second-order polynomial model with multiple regression. Analysis of variance was used to estimate model fitness and determine optimal conditions for processing. Estimated optimal conditions were 90% methanolic solution, 60 degrees C and 180min using these parameters; the predicted values of investigated responses were 43.19mg GAE/g (GAE: gallic acid equivalents), 43.04mg TE/g (TE: trolox equivalents), 139.34 and 93.76mg TE/g for TPC, DPPH, CUPRAC, and FRAP, respectively. The obtained optimal conditions could be considered as an alternative strategy for developing novel functional products. T2 - Analytical Letters T1 - Effects of Orange Leaves Extraction Conditions on Antioxidant and Phenolic Content: Optimization Using Response Surface Methodology EP - 1519 IS - 10 SP - 1505 VL - 51 DO - 10.1080/00032719.2017.1382501 UR - conv_715 ER -
@article{ author = "Uysal, Sengul and Cvetanović, Aleksandra and Zengin, Gokhan and Đurović, Saša and Zeković, Zoran and Aktumsek, Abdurrahman", year = "2018", abstract = "In the last few years, bioactive components or their extraction techniques are gaining special interest in scientific areas. In this framework, orange leaves were used for preparation of extracts with high content of biologically active compounds. To optimize the extraction process, three levels and three variables of Box-Behnken design with response surface methodology were applied. Investigated responses were the total phenolic content (TPC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, cupric ion reducing antioxidant capacity (CUPRAC), and ferric reducing antioxidant power (FRAP). Independent variables were methanol concentration (10-90%), temperature (20-60 degrees C), and extraction time (60-180min). Experimentally obtained results were fit into a second-order polynomial model with multiple regression. Analysis of variance was used to estimate model fitness and determine optimal conditions for processing. Estimated optimal conditions were 90% methanolic solution, 60 degrees C and 180min using these parameters; the predicted values of investigated responses were 43.19mg GAE/g (GAE: gallic acid equivalents), 43.04mg TE/g (TE: trolox equivalents), 139.34 and 93.76mg TE/g for TPC, DPPH, CUPRAC, and FRAP, respectively. The obtained optimal conditions could be considered as an alternative strategy for developing novel functional products.", journal = "Analytical Letters", title = "Effects of Orange Leaves Extraction Conditions on Antioxidant and Phenolic Content: Optimization Using Response Surface Methodology", pages = "1519-1505", number = "10", volume = "51", doi = "10.1080/00032719.2017.1382501", url = "conv_715" }
Uysal, S., Cvetanović, A., Zengin, G., Đurović, S., Zeković, Z.,& Aktumsek, A.. (2018). Effects of Orange Leaves Extraction Conditions on Antioxidant and Phenolic Content: Optimization Using Response Surface Methodology. in Analytical Letters, 51(10), 1505-1519. https://doi.org/10.1080/00032719.2017.1382501 conv_715
Uysal S, Cvetanović A, Zengin G, Đurović S, Zeković Z, Aktumsek A. Effects of Orange Leaves Extraction Conditions on Antioxidant and Phenolic Content: Optimization Using Response Surface Methodology. in Analytical Letters. 2018;51(10):1505-1519. doi:10.1080/00032719.2017.1382501 conv_715 .
Uysal, Sengul, Cvetanović, Aleksandra, Zengin, Gokhan, Đurović, Saša, Zeković, Zoran, Aktumsek, Abdurrahman, "Effects of Orange Leaves Extraction Conditions on Antioxidant and Phenolic Content: Optimization Using Response Surface Methodology" in Analytical Letters, 51, no. 10 (2018):1505-1519, https://doi.org/10.1080/00032719.2017.1382501 ., conv_715 .