Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts
2020
Аутори
Jovanović, MarinaMorić, Ivana
Nikolić, Biljana
Pavić, Aleksandar
Svircev, Emilija
Senerović, Lidija
Mitić-Ćulafić, Dragana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Many traditional remedies represent potential candidates for integration with modern medical practice, but credible data on their activities are often scarce. For the first time, the anti-virulence potential and the safety for human use of the ethanol extracts of two medicinal plants, Persicaria maculosa (PEM) and Bistorta officinalis (BIO), have been addressed. Ethanol extracts of both plants exhibited anti-virulence activity against the medically important opportunistic pathogen Pseudomonas aeruginosa. At the subinhibitory concentration of 50 mu g/mL, the extracts demonstrated a maximal inhibitory effect (approx. 50%) against biofilm formation, the highest reduction of pyocyanin production (47% for PEM and 59% for BIO) and completely halted the swarming motility of P. aeruginosa. Both extracts demonstrated better anti-quorum sensing and antibiofilm activities, and a better ability to interfere with LasR receptor, than the tested dominant extracts' constituents. The bioactive concentr...ations of the extracts were not toxic in the zebrafish model system. This study represents an initial step towards the integration of P. maculosa and B. officinalis for use in the treatment of Pseudomonas infections.
Кључне речи:
zebrafish / Persicaria maculosa / Bistorta officinalis / anti-quorum sensing activity / antibiofilm activityИзвор:
Molecules, 2020, 25, 8Издавач:
- MDPI AG
Финансирање / пројекти:
- Institute of General and Physical Chemistry, Serbia
- Биолошки активни природни производи као потенцијални извори нових лекова и дијететских суплемената (RS-MESTD-Basic Research (BR or ON)-172058)
- Изучавање микробиолошког диверзитета и карактеризација корисних срединских микроорганизама (RS-MESTD-Basic Research (BR or ON)-173048)
DOI: 10.3390/molecules25081811
ISSN: 1420-3049
PubMed: 32326481
WoS: 000534617300031
Scopus: 2-s2.0-85083675642
Институција/група
Institut za opštu i fizičku hemijuTY - JOUR AU - Jovanović, Marina AU - Morić, Ivana AU - Nikolić, Biljana AU - Pavić, Aleksandar AU - Svircev, Emilija AU - Senerović, Lidija AU - Mitić-Ćulafić, Dragana PY - 2020 UR - https://riofh.iofh.bg.ac.rs/handle/123456789/764 AB - Many traditional remedies represent potential candidates for integration with modern medical practice, but credible data on their activities are often scarce. For the first time, the anti-virulence potential and the safety for human use of the ethanol extracts of two medicinal plants, Persicaria maculosa (PEM) and Bistorta officinalis (BIO), have been addressed. Ethanol extracts of both plants exhibited anti-virulence activity against the medically important opportunistic pathogen Pseudomonas aeruginosa. At the subinhibitory concentration of 50 mu g/mL, the extracts demonstrated a maximal inhibitory effect (approx. 50%) against biofilm formation, the highest reduction of pyocyanin production (47% for PEM and 59% for BIO) and completely halted the swarming motility of P. aeruginosa. Both extracts demonstrated better anti-quorum sensing and antibiofilm activities, and a better ability to interfere with LasR receptor, than the tested dominant extracts' constituents. The bioactive concentrations of the extracts were not toxic in the zebrafish model system. This study represents an initial step towards the integration of P. maculosa and B. officinalis for use in the treatment of Pseudomonas infections. PB - MDPI AG T2 - Molecules T1 - Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts IS - 8 VL - 25 DO - 10.3390/molecules25081811 UR - conv_846 ER -
@article{ author = "Jovanović, Marina and Morić, Ivana and Nikolić, Biljana and Pavić, Aleksandar and Svircev, Emilija and Senerović, Lidija and Mitić-Ćulafić, Dragana", year = "2020", abstract = "Many traditional remedies represent potential candidates for integration with modern medical practice, but credible data on their activities are often scarce. For the first time, the anti-virulence potential and the safety for human use of the ethanol extracts of two medicinal plants, Persicaria maculosa (PEM) and Bistorta officinalis (BIO), have been addressed. Ethanol extracts of both plants exhibited anti-virulence activity against the medically important opportunistic pathogen Pseudomonas aeruginosa. At the subinhibitory concentration of 50 mu g/mL, the extracts demonstrated a maximal inhibitory effect (approx. 50%) against biofilm formation, the highest reduction of pyocyanin production (47% for PEM and 59% for BIO) and completely halted the swarming motility of P. aeruginosa. Both extracts demonstrated better anti-quorum sensing and antibiofilm activities, and a better ability to interfere with LasR receptor, than the tested dominant extracts' constituents. The bioactive concentrations of the extracts were not toxic in the zebrafish model system. This study represents an initial step towards the integration of P. maculosa and B. officinalis for use in the treatment of Pseudomonas infections.", publisher = "MDPI AG", journal = "Molecules", title = "Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts", number = "8", volume = "25", doi = "10.3390/molecules25081811", url = "conv_846" }
Jovanović, M., Morić, I., Nikolić, B., Pavić, A., Svircev, E., Senerović, L.,& Mitić-Ćulafić, D.. (2020). Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts. in Molecules MDPI AG., 25(8). https://doi.org/10.3390/molecules25081811 conv_846
Jovanović M, Morić I, Nikolić B, Pavić A, Svircev E, Senerović L, Mitić-Ćulafić D. Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts. in Molecules. 2020;25(8). doi:10.3390/molecules25081811 conv_846 .
Jovanović, Marina, Morić, Ivana, Nikolić, Biljana, Pavić, Aleksandar, Svircev, Emilija, Senerović, Lidija, Mitić-Ćulafić, Dragana, "Anti-Virulence Potential and In Vivo Toxicity of Persicaria maculosa and Bistorta officinalis Extracts" in Molecules, 25, no. 8 (2020), https://doi.org/10.3390/molecules25081811 ., conv_846 .