dc.creator | Simonović, Mladen | |
dc.creator | Mix, Thorsten | |
dc.creator | Glumac, Miodrag | |
dc.creator | Pejin, Boris | |
dc.date.accessioned | 2023-06-01T10:36:04Z | |
dc.date.available | 2023-06-01T10:36:04Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 1478-6419 | |
dc.identifier.uri | https://riofh.iofh.bg.ac.rs/handle/123456789/875 | |
dc.description.abstract | A new protein detector of 2,4,6-trinitrophenol (TNP, picric acid) is concisely reported herein for the first time. In brief, the gene of a specific scFv fragment, namely 3.5, was fused separately with the gene of beta-lactamase and, subsequently, expressed in the periplasmic space of Escherichia coli, affording in such a way the recombinant fusion protein 3.5-scFv-beta-lactamase. This bifunctional immunoreagent tool was further convincingly demonstrated for being capable to directly detect trinitrophenol-tris(hydroxymethyl)aminomethane (TNP-Tris), a less toxic TNP derivative of choice compared to TNP itself, with competitive sensitivity (50 +/- 2 fmol or 175 +/- 6 pg/mL). | en |
dc.publisher | Taylor & Francis Ltd, Abingdon | |
dc.relation | University Hamburg, Germany | |
dc.relation | German Academic Exchange Service (DAAD) | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172053/RS// | |
dc.rights | restrictedAccess | |
dc.source | Natural Product Research | |
dc.subject | TNP | en |
dc.subject | scFv-antibody | en |
dc.subject | new immunoreagent tool | en |
dc.subject | fusion protein (scFv-antibody  | en |
dc.subject | enzyme | en |
dc.subject | enzyme of choice) | en |
dc.subject | +  | en |
dc.title | 3.5-scFv-beta-lactamase, a new protein detector of picric acid, the highly nitrated organic explosive | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 1320 | |
dc.citation.issue | 5 | |
dc.citation.other | 36(5): 1317-1320 | |
dc.citation.rank | M22~ | |
dc.citation.spage | 1317 | |
dc.citation.volume | 36 | |
dc.identifier.doi | 10.1080/14786419.2020.1860977 | |
dc.identifier.pmid | 33336580 | |
dc.identifier.rcub | conv_896 | |
dc.identifier.scopus | 2-s2.0-85097738860 | |
dc.identifier.wos | 000599659300001 | |
dc.type.version | publishedVersion | |