Screening of Native Trichoderma Species for Nickel and Copper Bioremediation Potential Determined by FTIR and XRF
2023
Аутори
Racić, GordanaVukelić, Igor
Kordić, Branko
Radić, Danka
Lazović, Milana
Nešić, Ljiljana
Panković, Dejana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Soil pollution with heavy metals is a serious threat to the environment. However, soils polluted with heavy metals are considered good sources of native metal-resistant Trichoderma strains. Trichoderma spp. are free-living fungi commonly isolated from different ecosystems, establishing endophytic associations with plants. They have important ecological and biotechnological roles due to their production of a wide range of secondary metabolites, thus regulating plant growth and development or inducing resistance to plant pathogens. In this work we used indigenous Trichoderma strains that were previously isolated from different soil types to determine their tolerance to increased copper and nickel concentrations as well as mechanisms of metal removal. The concentrations of bioavailable metal concentrations were determined after extraction with diethylene-triamine pentaacetate (DTPA)-extractable metals (Cd, Cr, Co, Cu, Pb, Mn, Ni, and Zn) from the soil samples by inductively coupled plasma...-optical emission spectrometry (ICP-OES). Two indigenous T. harzianum strains were selected for copper tolerance, and three indigenous T. longibrachiatum strains were selected for nickel tolerance tests. Strains were isolated from the soils with the highest and among the lowest DTPA-extractable metal concentrations to determine whether the adaptation to different concentrations of metals affects the mechanisms of remediation. Mechanisms of metal removal were determined using Fourier-transform infrared spectroscopy (FTIR) and X-ray fluorescence spectroscopy (XRF), non-destructive methods characterized by high measurement speed with little or no need for sample preparation and very low costs. Increased DTPA-extractable metal content for nickel and copper was detected in the soil samples above the target value (TV), and for nickel above the soil remediation intervention values (SRIVs), for total metal concentrations which were previously determined. The SRIV is a threshold of metal concentrations indicating a serious soil contamination, thus confirming the need for soil remediation. The use of FTIR and XRF methods revealed that the presence of both biosorption and accumulation of metals in the Trichoderma cells, providing good bioremediation potential for Ni and Cu.
Кључне речи:
Trichoderma / nondestructive methods / heavy metals / biosorption / bioaccumulationИзвор:
Microorganisms, 2023, 11, 3Издавач:
- MDPI AG
Финансирање / пројекти:
- European Union [101079267]
- Provincial Secretary for Higher Education and Scientific Research, Autonomus Province of Vojvodina project [142-451-3172/2022-01/01]
DOI: 10.3390/microorganisms11030815
ISSN: 2076-2607
PubMed: 36985388
WoS: 000960176100001
Scopus: 2-s2.0-85151418059
Институција/група
Institut za opštu i fizičku hemijuTY - JOUR AU - Racić, Gordana AU - Vukelić, Igor AU - Kordić, Branko AU - Radić, Danka AU - Lazović, Milana AU - Nešić, Ljiljana AU - Panković, Dejana PY - 2023 UR - https://riofh.iofh.bg.ac.rs/handle/123456789/988 AB - Soil pollution with heavy metals is a serious threat to the environment. However, soils polluted with heavy metals are considered good sources of native metal-resistant Trichoderma strains. Trichoderma spp. are free-living fungi commonly isolated from different ecosystems, establishing endophytic associations with plants. They have important ecological and biotechnological roles due to their production of a wide range of secondary metabolites, thus regulating plant growth and development or inducing resistance to plant pathogens. In this work we used indigenous Trichoderma strains that were previously isolated from different soil types to determine their tolerance to increased copper and nickel concentrations as well as mechanisms of metal removal. The concentrations of bioavailable metal concentrations were determined after extraction with diethylene-triamine pentaacetate (DTPA)-extractable metals (Cd, Cr, Co, Cu, Pb, Mn, Ni, and Zn) from the soil samples by inductively coupled plasma-optical emission spectrometry (ICP-OES). Two indigenous T. harzianum strains were selected for copper tolerance, and three indigenous T. longibrachiatum strains were selected for nickel tolerance tests. Strains were isolated from the soils with the highest and among the lowest DTPA-extractable metal concentrations to determine whether the adaptation to different concentrations of metals affects the mechanisms of remediation. Mechanisms of metal removal were determined using Fourier-transform infrared spectroscopy (FTIR) and X-ray fluorescence spectroscopy (XRF), non-destructive methods characterized by high measurement speed with little or no need for sample preparation and very low costs. Increased DTPA-extractable metal content for nickel and copper was detected in the soil samples above the target value (TV), and for nickel above the soil remediation intervention values (SRIVs), for total metal concentrations which were previously determined. The SRIV is a threshold of metal concentrations indicating a serious soil contamination, thus confirming the need for soil remediation. The use of FTIR and XRF methods revealed that the presence of both biosorption and accumulation of metals in the Trichoderma cells, providing good bioremediation potential for Ni and Cu. PB - MDPI AG T2 - Microorganisms T1 - Screening of Native Trichoderma Species for Nickel and Copper Bioremediation Potential Determined by FTIR and XRF IS - 3 VL - 11 DO - 10.3390/microorganisms11030815 UR - conv_1096 ER -
@article{ author = "Racić, Gordana and Vukelić, Igor and Kordić, Branko and Radić, Danka and Lazović, Milana and Nešić, Ljiljana and Panković, Dejana", year = "2023", abstract = "Soil pollution with heavy metals is a serious threat to the environment. However, soils polluted with heavy metals are considered good sources of native metal-resistant Trichoderma strains. Trichoderma spp. are free-living fungi commonly isolated from different ecosystems, establishing endophytic associations with plants. They have important ecological and biotechnological roles due to their production of a wide range of secondary metabolites, thus regulating plant growth and development or inducing resistance to plant pathogens. In this work we used indigenous Trichoderma strains that were previously isolated from different soil types to determine their tolerance to increased copper and nickel concentrations as well as mechanisms of metal removal. The concentrations of bioavailable metal concentrations were determined after extraction with diethylene-triamine pentaacetate (DTPA)-extractable metals (Cd, Cr, Co, Cu, Pb, Mn, Ni, and Zn) from the soil samples by inductively coupled plasma-optical emission spectrometry (ICP-OES). Two indigenous T. harzianum strains were selected for copper tolerance, and three indigenous T. longibrachiatum strains were selected for nickel tolerance tests. Strains were isolated from the soils with the highest and among the lowest DTPA-extractable metal concentrations to determine whether the adaptation to different concentrations of metals affects the mechanisms of remediation. Mechanisms of metal removal were determined using Fourier-transform infrared spectroscopy (FTIR) and X-ray fluorescence spectroscopy (XRF), non-destructive methods characterized by high measurement speed with little or no need for sample preparation and very low costs. Increased DTPA-extractable metal content for nickel and copper was detected in the soil samples above the target value (TV), and for nickel above the soil remediation intervention values (SRIVs), for total metal concentrations which were previously determined. The SRIV is a threshold of metal concentrations indicating a serious soil contamination, thus confirming the need for soil remediation. The use of FTIR and XRF methods revealed that the presence of both biosorption and accumulation of metals in the Trichoderma cells, providing good bioremediation potential for Ni and Cu.", publisher = "MDPI AG", journal = "Microorganisms", title = "Screening of Native Trichoderma Species for Nickel and Copper Bioremediation Potential Determined by FTIR and XRF", number = "3", volume = "11", doi = "10.3390/microorganisms11030815", url = "conv_1096" }
Racić, G., Vukelić, I., Kordić, B., Radić, D., Lazović, M., Nešić, L.,& Panković, D.. (2023). Screening of Native Trichoderma Species for Nickel and Copper Bioremediation Potential Determined by FTIR and XRF. in Microorganisms MDPI AG., 11(3). https://doi.org/10.3390/microorganisms11030815 conv_1096
Racić G, Vukelić I, Kordić B, Radić D, Lazović M, Nešić L, Panković D. Screening of Native Trichoderma Species for Nickel and Copper Bioremediation Potential Determined by FTIR and XRF. in Microorganisms. 2023;11(3). doi:10.3390/microorganisms11030815 conv_1096 .
Racić, Gordana, Vukelić, Igor, Kordić, Branko, Radić, Danka, Lazović, Milana, Nešić, Ljiljana, Panković, Dejana, "Screening of Native Trichoderma Species for Nickel and Copper Bioremediation Potential Determined by FTIR and XRF" in Microorganisms, 11, no. 3 (2023), https://doi.org/10.3390/microorganisms11030815 ., conv_1096 .